Gulmai Irani was born in 1882 in Parel, Bombay. In December 1896 she married Kaikhushru Sarosh Irani, a prominent businessman and civic leader of Ahmednagar, and they had four children: Rustom, Adi, Piroja and Dolly. For years she was depressed and unhappy in her husband's joint-family household, where her religious devotion was ridiculed by her in-laws.
In August 1919 her younger sister Soonamasi and Soonamasi's husband Kaikhushru Masa persuaded her, after two weeks of pleading, to visit the Perfect Master Upasni Maharaj at Sakori. On entering his hut she was astonished to recognize him as the figure who had recently appeared to her in a vivid dream. That same day she met the young Merwan Seth (Meher Baba), who remarked how different his life would have been had she been his mother. Over the following years she visited Sakori often and endured severe tests there; Merwan assured her that by withstanding them she had become the first woman to be granted the grace of a Perfect Master such as Upasni Maharaj.
In 1921 at Sakori, Meher Baba told her that every Perfect Master has a spiritual mother and that she was his. Upasni Maharaj made it clear that she should defer to Meher Baba in all matters. That July, against the objections of orthodox relatives, she insisted that Upasni Maharaj himself open the family's new house, Sarosh Manzil, in Ahmednagar.
When Baba spoke in 1923 of moving to a village, Gulmai invited him to the family's property near Arangaon, outside Ahmednagar. That land became Meherabad, and she and her husband gave it to him. During the 1920s she and her son Adi often carried messages between Sakori and Meherabad. In May 1924, when Baba was about to leave Meherabad after a family quarrel, her tears moved him to stay, and he said he had stepped out of the car only because of Mother.
Gulmai supported the ashram from Khushru Quarters in Ahmednagar, sending supplies and coming daily to share the work of the women mandali; for a period she also lived with them on Meherabad Hill. Her sons Adi and Rustom served Baba for life, and in 1945 her husband, long respectful but distant, was finally drawn to Baba shortly before his death.
In August 1962, gravely ill with cancer and kidney disease, Gulmai was visited by Baba on the 9th; though nearly unconscious, she caressed his face and whispered his name. She died at midnight on 10 August 1962, calling out Baba's name. The next morning Baba himself attended her burial on Meherabad Hill, where she became the first person to be buried. It was the last time Meher Baba went to Meherabad. Meher Baba Travels gives her birth year as 1882; Lord Meher gives her age as 36 in 1919 and 78 at her death.